The focus of this Creative Inquiry project is to study the biophysics of Trypanosoma brucei for the purpose of understanding the molecular mechanism of the flagella in order to develop treatments for kinetoplastid diseases. The kinetoplastid T. brucei was selected due to the unique wave the flagella exhibits, which propagates from tip-to-base rather than base-to-tip, as observed in other eukaryotes. Our hypothesis is that this movement is due to the coordination mechanism of axonemal dynein motor proteins. We are studying this through the knockdown and tagging of the axonemal dynein.
In order to more thoroughly complete in-depth research, we have divided the project into three subcategories:
- RNA Knockdown
- Fluorescent Imaging
- Biophysical Analysis/Scale-Up
- Post Translational Modifications of Tubulin
For details, see the Trypanosome Biophysics CI's web page.